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Page 35
Volume 3
Microbiology 2019 & Fungal infections 2019
October 07-08, 2019
Journal of Clinical Microbiology and Infectious diseases
October 07-08, 2019 | Madrid, Spain
MICROBIOLOGY AND MICROBIOLOGISTS
MYCOLOGY AND FUNGAL INFECTIONS
2
nd
Annual Congress on
6
th
International Conference on
&
J Clin Microbiol Infect Dis, Volume 3
CorrelationbetweenBiofilmFormationandthepresenceofextendedspectrumβ-lactamase
bla
PER1
gene in multiple drug resistant clinical isolates of
Acinetobacter baumannii
Nouran Magdy Mohamed Moustafa
Dar Al Uloom University, KSA
Statement of the Problem
:
Acinetobacter baumannii
(
A. baumannii
) is responsible for most hospital acquired infections. Among the
most important
A. baumannii
virulence factors, is its ability to produce biofilm, which relates to its high degree of antibiotic resistance
and survival in hospital environment.
The purpose of this study is to identify the ability of different
A. baumannii
isolates to produce biofilm, and whether this correlates to
multidrug resistance and the presence of blaPER-1gene.
Methodology & Theoretical Orientation
: A total of 30 isolates of
A. baumannii
were subjected to susceptibility testing by disc
diffusion method for 10 clinically relevant antibiotics followed by phenotypic detection of ESBL production by double disc synergy
test (DDST). MIC for imipenem was performed by E-test. Screening for biofilm formation was done by microtitre plate assay. The
presence of blaPER-1 was investigated by PCR.
Finding
:
A. baumannii
isolates showed high rate of resistance to the tested antimicrobials. 97.6% were ESBL producers. Sixteen
isolates (53.3%) were biofilm producers. There was no significant relation between biofilm formation and MDR. blaPER-1 gene was
detected in fifteen isolates (50%) but showed non-significant correlation with biofilm formation and ESBL production.
Conclusion
: This study demonstrates the increasing likelihood of
A. baumannii
isolates to form biofilms. There is no significant
association between biofilm formation, MDR and blaPER-1 gene.
nouran.m@dau.edu.sa