2 / 14
Page 23
Volume 3
Microbiology 2019 & Fungal infections 2019
October 07-08, 2019
Journal of Clinical Microbiology and Infectious diseases
October 07-08, 2019 | Madrid, Spain
MICROBIOLOGY AND MICROBIOLOGISTS
MYCOLOGY AND FUNGAL INFECTIONS
2
nd
Annual Congress on
6
th
International Conference on
&
J Clin Microbiol Infect Dis, Volume 3
Aetiology and clinical profile of acute bacterial meningitis in children less than 12 year
admitted at a tertiary care hospital in North India
Jyotsna Agarwal, Palak Mehrotra, Sugandha Srivastaava
and
Mala Kumar
Dr. Ram Manohar Lohia Institute of Medical Sciences, India
A
cute Bacterial meningitis (ABM) is amongst most common causes of morbidity & mortality in children in developing
countries; delay in diagnosis and initiation of inappropriate antibiotics further adds to fatal outcome or long-term
neurological sequelae. The present prospective observational study was conducted to know the etiology and epidemiologic
risk factors associated with ABM among children in a tertiary care hospital of North India to ensure early and appropriate
management. Out of total 225 patients enrolled, 118/225 patients fulfilled criteria of Nelson book of Pediatrics and WHO were
enrolled in NICU (age group ranges from day 1 to ≤4 weeks), whereas 107/225 patients were enrolled from CHDS (age group
ranges from> 4 weeks to 12 year); respectively. For each suspected case, demographic data, predominant clinical signs and
symptoms, prior history of use of antimicrobial agent, and laboratory results was recorded in pre-designed questionnaire. CSF
samples were subjected to direct wet mount, Gram staining and bacterial culture followed by Antimicrobial sensitivity testing
(AST) by Kirby Bauer disk diffusion method and results were interpreted as per CLSI (2015). Mean age of both NICU and CHDS
cases were 7±6.9 days and 4.94 ±3.3 years with female: male ratio as 1.87:1 and 1.60:1 respectively. In NICU patients, elevated
CSF protein (>100mg/dl) was present in 31.3% and decreased CSF glucose (<40 mg/dl) were found in 61.8% whereas 80.5%
had increased cell count and were significantly associated with culture positivity (p=0.001 and p= 0.008), respectively. Most
common predisposing factors in mother was maternal fever (47.7%), leaky per vagina (40.9). Our result showed that
S. aureus
,
E. coli
,
Enterococcus faecali
and
CoNS
were highly predominat gram positive bacteria in children age >12 years followed by
gram negative bacteria
Acinetobacter spp, Klebseilla pneumoniae, E. coli
and
Pseudomonas aerouginosa
. Proper vaccination
for
N. Meningitides, S. Pneumoniae and H. Influenzae
type b in developing countries have shown the less predominancy of
these pathogens isolated fromABM. Antibiotic suspetibility pattern of showed that
Gram positive cocci
were mostly sensitive to
Vancomycin, linezolid followed by amikacin and gentamycin. All GNBs isolated in the present study were sensitive to colistin
followed by meropenem and imipenem. Hence, this type of studies should be done on large scales to gather data for formulation
of regional disease specific policies.
Biography
Jyotsna Agarwal is working in the area of patient care and diagnostics. Currently she is working as Head in the Department of
Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences. Her thrust areas of interest are sexually transmitted/ reproductive
tract/urinary tract infections in women. Her other research interests are antimicrobial resistance in microbes, molecular diagnostics
and focus areas have been infections of children like pneumonia, septicemia & meningitis. She has nearly 60 publications in indexed
national and international journals and also worked as Editor and Reviewer for several reputed National and International journals. She
took keen interest in “Hospital Infection Prevention, Control Practices and Antimicrobial stewardship Program” and provides advice
on prevention of misuse of antimicrobials in hospital settings. An Annual newsletter from the Department of Microbiology, Dr. Ram
Manohar Lohia Institute of Medical Sciences is published in her guidance, which contains antibiograms and other relevant information
pertaining to infection control etc.
jyotsnaagarwal.micro@gmail.com