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Volume 3 Journal of Neurology and Clinical Neuroscience
Neurosurgery 2019 & Neuroimmunology 2019
May 22-23, 2019
Page 12
Neurosurgery and Neurological Surgeons
Neuroscience and Neuroimmunology
May 22-23, 2019 London, UK
6 th Annual Meeting on
9 th Global Summit on
&
J Neurol Clin Neurosci, Volume 3
Neuroprotective and regenerative roles of the Wnt-3A pathway after focal ischemic stroke in mice
Wnt signaling is a conserved pathway involved
in expansion of neural progenitors and
lineage specification during development. However,
the role of Wnt signaling in the post-stroke brain
has not been well- elucidated. We hypothesized
that Wnt-3a would play an important role for
neurogenesis and brain repair. Adult male mice
were subjected to a focal ischemic stroke targeting
the sensorimotor cortex. Mice that received Wnt-3a
(2 µg/kg/day, 1 hr after stroke and once a day for
the next 2 days, intranasal delivery) had reduced
infarct volume compared to stroke controls. Wnt-
3a intranasal treatment of 7- days upregulated
the expression of brain-derived growth factor
(BDNF), increased the proliferation and migration
of neuroblasts from the subventricular zone (SVZ),
resulting in increased numbers of newly formed
neurons and endothelial cells in the penumbra. Both
the molecular and cellular effects of Wnt-3a were
blocked by the Wnt specific inhibitors XAV-939 and
Dkk-1. In functional assays, Wnt-3a treatment enhanced the local cerebral blood flow (LCBF) in the penumbra, as well as
improved sensorimotor functions in a battery of behavioral tests. Together, our data demonstrates that Wnt-3a signaling
can act as a dual neuroprotective and regenerative factor for the treatment of ischemic stroke.
Biography
Taylor joined the faculty as an Assistant Professor of Genetics in the Department of Biology in the College of Science, Engineering, and
Technology at Jackson State University. While at JSU, Dr. Taylor is a Research Center f or Minority Investigators (RCMI) faculty member,
a graduate and undergraduate student advisor, mentor, and professor. She obtained her PhD in Microbiology from Indiana University,
a Masters of Science degree in Biology, from Jackson State University, and her Bachelors of Science degree in Biology f rom Tougaloo
College. She was a Fellowship in Research and Science Teaching (FIRST) postdoctoral fellowship at Emory University in the Departments
of Anesthesiology and Neurology. Her current research focus is determining the role of signal transducer activator of transcription 3 (STAT-3)
on the regeneration of nerve tissue and functional recovery after focal ischemic stroke.
Tammi Taylor
Jackson State University, USA
Figure 3. Downregulation of Wnt activity downregulated endogenous neurogenesis after stroke. A.
Representativ e immunof luorescence images f or NeuN+ (green), BrdU+ (red), and NeuN+BrdU+
colabeled cells (white arrows) among dif f erent treatment groups. B. Representativ e conf ocal
3-dimensional image (Z-s t a c k thickness = 10 Bm) conf irming colocalization of BrdU and NeuN f
luorescence. C. Quantif ication of neurogenesis by NeuN+BrdU+ colabeled cells in the penumbra f
ollowingadministrationofeithersaline(negativecontrol)ortheWnt-signalinginhibitor,XAV-939.All
datarepresentedasmean•}SEM;p0.05comparedtosham;#p0.05comparedtosaline.N=4-10group.