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Volume 02
Stem Cells 2019 & Pediatrics Congress 2019
November 06-07, 2019
Journal of Clinical Genetics and Genomics
November 06-07, 2019 | Tokyo, Japan
STEM CELLS AND REGENERATIVE MEDICINE
PEDIATRICS AND CHILD CARE
International Conference on
2
nd
World Congress on
&
J Clin Gen Genomics, Volume 02
Gradient photothermal field for precisely directing cell sheet detachment
Qinghua Lu
Shanghai Jiao Tong University, China
T
raditional cell-based medical therapeutics are usually performed with the enzymes (trypsin) to transfer cells from culture dishes to
the wounds, which destroy the extracellular matrix (ECM) and reduce cell efficacy, cell activity and cell integrity. Technologies
for transferring cell sheets with a completely integrated ECM and intact cell–cell junctions have been established for applications in
surgical operations and regenerative medicine, such as ocular regeneration, cardiac tissue regeneration and bladder augmentation.
Developments of cell sheet engineering are in progress aimed at commercial applications. However, there are still a lot of problems in
terms of practical operation. For example, the resultant cell sheets are easily crumpled, wrinkled and tangled due to the uncontrollable
detachment and disturbance from hydrodynamic forces. For more delicate clinical trials, a
unifying cell sheet operation with careful detaching direction could facilitate consistent,
ordered and reproducible production. Strategies to harvest cell sheets along a predetermined
direction are significant to address this issue but still in the infancy.
A PEDOT film with gradient thickness was designed to provide a gradient photothermal field
for locally dissolving a type I collagen layer. Type I collagen is comprised of cross-linked
tri-helical peptides and forms a gel at relatively low temperature, which promptly dissolves
on heating beyond 42°C. Its dissociation rate is linearly related to temperature. Here, under
a gradient photothermal field, precisely directed collagen dissolution was expected to guide
detachment of the cell sheet. Intuitively, as this novel scenario provides an opportunity for
precisely directing cell sheet detachment, it can be envisaged as greatly facilitating broader
commercial application of next-generation cell sheet engineering.
qhlu@sjtu.edu.cnFigure 1. Scheme of gradient photothermal
surface for precisely directing cell sheet
detachment