stem-cells-2019-posters-abstracts

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Volume 02

Stem Cells 2019 & Pediatrics Congress 2019

November 06-07, 2019

Journal of Clinical Genetics and Genomics

November 06-07, 2019 | Tokyo, Japan

STEM CELLS AND REGENERATIVE MEDICINE

PEDIATRICS AND CHILD CARE

International Conference on

World Congress on

J Clin Gen Genomics, Volume 02

Protective effects of uncultured adipose derived stromal vascular fraction on testicular

injury

Ruipeng Jia

Nanjing First Hospital, Nanjing Medical University, China

Introduction

: Torsion-detorsion (T/D) induced testicular injury may lead to male subfertility and even infertility. Stem cell therapy

provides an alternative to attenuate testicular injury and promote spermatogenesis. Adipose derived stromal vascular fraction (SVF)

can be acquired conveniently without

in vitro

expansion, which may avoid the potential risks of microbial contamination, xenogenic

nutritional sources, etc., during cell culture. In this study, we investigate the protective effects of autologous uncultured SVF on

testicular injury and spermatogenesis in a rat model of T/D.

Methods

: Animals were randomly divided into sham, T/D+ phosphate-buffered saline (PBS) and T/D+SVF groups (eighteen rats

in each group). SVF was isolated, labeled with lipophilic fluorochrome chloromethylbenzamido dialkylcarbocyanine (CM-DiI) and

transplanted into T/D testis by local injection. At 3, 7, 14 and 28 days after surgery, testicular tissue and serum samples were harvested

for histopathological, immunohistochemical, Western blot and enzyme-linked immunosorbent assay.

Results

: Histopathological findings demonstrated severe injury in testis with decreased Johnsen’s score led by T/D, while uncultured

SVF reduced testicular injury and elevated the decreased score. Injected SVF cells were mainly integrated into interstitial region and

seminiferous tubules, enhanced the secretion of basic fibroblast growth factor and stem cell factor in testis, contributed to the declining

level of malondialdehyde and restoration of hormonal homeostasis and then reduced the injury of Leydig cells and germ cells, as well

as promoting spermatogenesis.

Conclusion

: Our findings demonstrated that autologous uncultured SVF could protect testis from testicular I/R injury and promote

spermatogenesis, which provide significant clinical implications for the prevention of infertility induced by testicular T/D.