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Volume 02
Clinical Pharmacology and Toxicology Research
Toxicology 2019
November 11-12, 2019
November 11-12, 2019 | London, UK
TOXICOLOGY AND CLINICAL TOXICOLOGY
2
nd
International Conference on
Clin Pharmacol Toxicol Res, Volume 02
Protective effect of Baicalein alone and co-administeredwithLosartan onDoxorubicin-
induced Nephrotoxicity in rats
Ziad H Al-Oanzi
Jouf University, Saudi Arabia
D
oxorubicin (DOX) is a widely used antineoplastic drug with several toxic effects. We investigated
the protective effect of co-administration of Baicalein (BL; a flavonoid) and losartan (LT;
angiotensin receptor blocker) on DOX-induced nephrotoxicity. Male Wistar albino rats were divided
into these seven groups (n=6): 1) Control group; 2) DOX group; 3) DOX+BL group (BL, 10 mg/kg/
day); 4) DOX+LT group (LT, 7 mg/kg/day) and 5) DOX+LT+BL(10) group. After two weeks of LT
and BL treatment, a dose of DOX was administered. Serum renal markers such as creatinine and urea
levels were significant (P <0.001) elevated in DOX challenged group compared to normal animals.
Renal pro-inflammatory cytokines including tumour necrosis factor-α (TNFα), interleukin (IL-1β and
IL-6) levels were significantly (P<0.001) increased while an anti-inflammatory cytokine IL-10 levels
were markedly (P<0.001) increased in DOX challenged group compared to controls. Oxidative stress
biomarkers including thiobarbituric acid reaction substances (TBARS) and glutathione (GSH) in renal
cells were significantly (P<0.001) increased and decreased compared to control group respectively.
Enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)
and glutathione-S-transferase (GST) in renal cells were significantly (P<0.01) decreased in DOX
challenged rats compared to normal. In addition, renal protein expressions and inflammatory activities
of caspase-3, n-nitric oxide syntheses (nNOS), inducible nitric oxide synthase (iNOS), endothelial nitric
oxide synthase(eNOS) and nuclear factor kappa-B (NF-κB) p65 were significantly (P<0.001) increased
in DOX challenged rats when compared to control animals. While the DOX-induced increase in serum renal markers, pro-
inflammatory cytokines and biomarkers was alleviated by BL and/or LT treatment and showed the most potent protective effects.
Our study demonstrates remarkable anti-oxidative and anti-inflammatory effects of BL and LT in rodents challenged with DOX.
Biography
Ziad H Al-Oanzi has graduated from Institute of Cellular Medicine, The Medical Science University of Newcastle (UK), has PhD was
focusing on The role of the hexosamine biosynthesis pathway in control of hepatic glucose metabolism. His current research interests
are biochemistry of liver, glucose and glycogen metabolism, metabolism control in gene expression, inflammation and antioxidants. He
is working as an assistant professor at the Jouf University, College of Applied Medical Science, Sakaka, Saudi Arabia.
e
:
zhaloanzi@ju.edu.sa