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Page 23

Volume 3

August 5-6, 2019 | Singapore

CANCER RESEARCH AND PHARMACOLOGY

STRUCTURAL BIOCHEMISTRY, STEM CELLS AND MOLECULAR BIOLOGY

24

th

International Conference on

International Congress on

&

Cancer Research 2019 & Structural Biochemistry 2019

August 5-6, 2019

Journal of Cancer and Metastasis Research

Clin Psychol Cog Sci, Volume 3

Chitinase production from

Paenibacillus sp

. BISR-047 utilizing seafood waste as

substrate under solid-state fermentation

Saavi Pradhan

Banasthali University, India

C

hitinases have huge potential applications and biological value in industries; they are used in generating single cell proteins,

sweeteners, insecticides, antifungal drugs, anti cancer agents, biopesticides, food processing agents, degrading agents for

sea waste etc. Chitinases are used for the conversion of chitin a polysaccharide into monomers. Extraction of chitin involves

two steps, demineralisation and deproteinisation, which can be conducted by two methods, chemical or biological. The chemical

method requires the use of acids and bases, while the biological method involves microorganisms.

Solid-state fermentation (SSF) is a low-cost fermentation technology, particularly suitable for the needs of developing countries.

This bioconversion technology of chitinous materials through chitinolytic process is an alternative waste treatment that not only

solves environmental problems but also decreases the production costs of microbial chitinases. Therefore, efforts were made in

the present study to utilize seafood waste for chitinase production under SSF. A novel thermo-tolerant bacterium Paenibacillus

sp. BISR-047, previously isolated from the Great Indian Desert soils, was used and various process parameters were studied.

We obtained 346 IU/ml of chitinase production in a medium containing crab and prawn waste (5:2; waste: water), 1.5 g/kg

yeast extract (w/w), 0.5 g/kg NaCl (w/w), 40% moisture content, pH 8 and at 45 ºC temperature. We obtained 29% dry weight

reduction after 10 d of incubation under SSF. Our results indicate scope for the utilization of seafood waste for industrial

production of chitinase using SSF.

Biography

Saavi Pradhan has her expertise in the field of Microbiology and analytical research studies with experience contribution in

microbiological studies of Great Indian Desert soils. Phytopathogens which cause severe damage to commercial crops were also

studied and successful field trials have been done. Her work also involved pest control biological methodologies to overcome disease

causing pest issues.

sjorwal88@gmail.com