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Volume 2 Journal of Clinical Diagnosis and Treatment Annual Nephrology & Chronic Diseases 2019
May 20-21, 2019
Nephrology
Chronic Diseases May 20-21, 2019 London, UK
19th Annual Conference on
3rd International Conference on
&
IgG1 anti-PC reduces CVD risk in SLE patients by efficiently clearing apoptotic cells
Thiagarajan D, Frostegård A and Frostegård J
Karolinska Institutet, Sweden
Background: Dual role of natural IgM antibodies against both phosphorylcholine (PC) and Malondialdehyde
(MDA) in assisting the clearance of apoptotic cells and reducing atherosclerotic plaques size has been suggested
previously. In this study we aim to understand the role of IgG1, IgG2 in Cardiovascular outcomes in SLE and
their functions in in-vitro conditions.
Methods: We used a unique SLE- Vascular Impact Cohort (SLEVIC), which consisted of 116 SLE-patients
from Karolinska University Hospital Huddinge and 110 matched controls. The levels of IgG1s and IgG2 was
measured by ELISA. For functional studies, we used fully human monoclonal antibodies. Primary human
macrophages were cultured with MCSF. Apoptosis was induced in TAMRA labelled Jurkat T-cells by CD95
ligand and incubated with anti-PC IgGs or isotype control IgG1 (1μg/mL), one hour prior to phagocytosis.
Anti-PC antibody labelled apoptotic Jurkat cells were fed to mature M2 macrophages for 60-80 mins and the
cells were formalin-fixed for microscopic examination. Phagocytosis was assessed by number of macrophages
up-taking TAMRA labelled Jurkat cells to the total number of macrophages in the given area.
Results: IgG1 Anti-PC and IgG2 Anti-MDA were negatively associated with atherosclerosis IgG1 Anti-PC was
negatively associated with CVD, SLEDAI and SLICC Simultaneously, the IgG1 anti-PC clones D05, improved
phagocytosis efficiency from 25% (both macrophages and isotype control) to and 53%(p=0,007) followed by
E01, 37%(p=0,05) and A01,28% (p=NS) where, the anti-PC IgG1s bind to phosphorylcholine exposed on
apoptotic cells and facilitate the uptake by macrophage though Fc gamma receptor.
Conclusion: The impaired clearance of apoptotic cells and increased OxLDL uptake are hallmark in both
SLE and development of necrotic core in atherosclerotic plaques respectively. This study evidently show that
anti-PC IgG1 is not only lower in atherosclerotic patients but exogenous addition of anti-PC IgG1s efficiently
improves the apoptotic cell uptake thereby making a possible therapeutic candidate for SLE patients in future.
J Clinical Diagnosis and Treatment, Volume 2