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Volume 02
Stem Cells 2019 & Pediatrics Congress 2019
November 06-07, 2019
Journal of Clinical Genetics and Genomics
November 06-07, 2019 | Tokyo, Japan
STEM CELLS AND REGENERATIVE MEDICINE
PEDIATRICS AND CHILD CARE
International Conference on
2
nd
World Congress on
&
J Clin Gen Genomics, Volume 02
Pentaisomaltose- a promising new cryoprotectant
Jesper Dyrendom Svalgaard
University of Copenhagen, Denmark
Cell and tissue therapy for medical and scientific use has during the past decades expanded extensively and hold promises for
being an important platform for future treatment modalities. A major feature and pre-requisite for several cell products, both
autologous and allogeneic off-the shelf, is the possibility of safe and efficient cryopreservation during production/treatment.
Successful cryopreservation requires effective cryoprotectants (CPAs). Classical and CPAs like glycerol and especially dimethyl
sulfoxide (DMSO) have been widely used. However, the well-established side-effects and undesirable cellular effects of e.g.
DMSO, has led to an increasing demand from health care professionals, patients and authorities for a reduction in the concentration
of CPAs like DMSO and/or the development of safe and effective alternatives. Most development in the past decades has focused
on optimizing freeze media, by combining existing CPAs and optimizing the carrier media, but new alternative CPAs have not
been introduced.
Pentaisomaltose, a low-molecular-weight carbohydrate, is a new and promising CPA. Our work during the past years has proven
that pentaisomaltose can replace DMSO for cryopreservation of hematopoietic stem cells and, also to significantly reduce the
required concentration of DMSO needed for successful cryopreservation of other cell types (ASC, T-Cells). Pentaisomaltose,
thus, could prove a promising new addition to the CPA repertoire, solving some of the problems faced by the field today.
Biography
Jesper Dyrendom Svalgaard is MSc, PhD and research fellow in the stem cell facility, at the Department of Clinical Immunology,
Rigshospitalet, University of Copenhagen. In his research Jesper D Svalgaard is focusing on the development and testing of alternative
cryoprotectants for cryopreservation of hematopoietic stem cells products and other cell types. Lately, part of his research interests has
been testing how to reduce the DMSO concentration in mesenchymal stem cells and T cells.
Jesper.Dyrendom.Svalgaard@regionh.dk