biotechnology-2018-posters-abstracts

Page 39

Volume 2

Journal of Medical Biotechnology

Biotechnology 2018

July 16-17, 2018

World Biotechnology Congress

July 16-17, 2018 Berlin, Germany

J Med Biotechnol 2018, Volume 2

Evaluation of the efficacy of recombinant pollen and fruit allergens by ELISA and immune-blotting

Mohammad Fereidouni and Afshin Derakhshani

Birjand University of Medical Sciences, Iran

Introduction & Aim:

Allergenic extracts from different sources extensively use for the diagnosis and treatment of allergic disorders.

However, the potency and stability of commercial natural extract is vary and in some cases dramatically decrease by time mostly

due to inherent enzyme activities producing recombinant allergens is proposed as an alternative method which can provide enough

allergens and improves stability and potency of allergens. Among different methods for expressing the allergenic proteins, the

E. coli

system is the first-choice for the initial screening of as it can be easily manipulated, cultured inexpensively and grows rapidly. The aim

of this study was to evaluate the potency of some recombinant allergens with natural extract by ELISA and immune-blotting methods.

Method:

Recombinant major allergens of melon, grape,

Chenopodium album

and

Salsola kali

pollen were expressed in

E. coli

lyophilized and purified by gel extraction. Total protein content was measured by Bradford method and after adjustment of the

concentration with commercial natural extract; the potency of extracts was assessed by ELISA and immune-blotting methods using

patients’ sera.

Results:

The potency of pollen recombinant allergens was comparable with natural extracts but for grape and melon, the potency

was better than natural extracts. With increasing the concentration of major allergens in extracts, the potency of extracts increased

accordingly.

Conclusion:

Recombinant allergens particularly for fruits which are unstable are the suitable alternative sources and can use in

vitro

and

in vivo

diagnostic methods for allergy evaluation.