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Volume 2
Journal of Medical Biotechnology
Biotechnology 2018
July 16-17, 2018
World Biotechnology Congress
July 16-17, 2018 Berlin, Germany
Phenotypic and proteomic evaluation of EMS-induced mutants of Nigella sativa
Ambreen Asif and M Yunus Khalil Ansari
Aligarh Muslim University, India
N
igella sativa
L. (Black cumin) is a small herbaceous plant, belonging to family Ranunculaceae. Seeds of this plant have huge
medicinal properties. However, the low seed yield is not fulfilling the demand for pharmaceutical purposes. Therefore, an
attempt has been made in this study to develop mutant lines of
Nigella sativa
by using EMS. It has been found that 0.1% EMS
resulted in the mutant line which is taller with pro-fused branching with high yield compared to wild type plant. Interestingly
1.0% EMS treatment produced dwarf mutant with few branches and low yield. To investigate the contrasting character of both
the mutants, proteome analysis of leaves was carried out through 2D-gel electrophoresis. Analysis of proteome of wild type and
mutant lines showed that 32 proteins were differentially expressed. These differentially expressed proteins were sequenced through
MALDI-TOF and identified by using MASCOT software. The function and location of these proteins were analyzed by using Uniprot
software. These proteins were categorized on the basis of their functions and correlated with the phenotypic characters of mutant
lines. Validation of differentially expressed proteins was carried out through studying expression pattern of respective gene through
quantitative real time PCR. It has been found that expression of 26 proteins was due to mutation in the mutant lines.
Biography
Ambreen Asif is attending the University of Aberdeen, Scotland, United Kingdom for an Advance Research in Genetic Mapping after being awarded the prestigious
Newton-Bhabha Research Fellowship. She is pursuing PhD in AMU with specialization in Genetics and Cytogenetics. She has earlier been awarded the INSPIRE
Research Fellowship of Department of Science & Technology (DST), Government of India. She has also been conferred with Anjalina Khan Award and Regularity
Award by the Women’s College in AMU.
mbreenasif1@gmail.comAmbreen Asif et al., J Med Biotechnol 2018, Volume 2