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Page 36
Volume 2
Journal of Medical Biotechnology
Biotechnology 2018
July 16-17, 2018
World Biotechnology Congress
July 16-17, 2018 Berlin, Germany
J Med Biotechnol 2018, Volume 2
Identification of cis-regulatory elements of butyrophilin gene of the mammary gland
Demie Aschalew Lemma
1
, Zegeye Abiy
2
, Qayyum Khan Abdul
3
1
Bioinformatics and Genomics
2
Addis Ababa University, Ethiopia
3
Arba Minch University, Ethiopia
B
utyrophilin subfamily 1 member A1 is a highly expressed gene in mammary gland of all mammals during lactation. It is found
to be the major integral protein in the milk fat globule membrane. Its interactions with other membrane elements and soluble
protein of the mammary epithelial cells regulate the secretion of milk fat. In order to reveal any shared cis-acting elements, the
human butyrophilin subfamily 1member A1 5’ genomic sequence was analyzed and compared with four mammals comprising of a
rodent and three primates, viz., Mus musculus Macaca mulata, and three other mammary specific human milk genes using publicly
available bioinformatics tools. Prior to a multiple sequence analysis, low complexity DNA sequences were masked using CENSOR.
The multiple sequence analysis revealed nine highly conserved regions of similarities in the 5’ butyrophilin genes across species.
Consensus putative transcription factor binding sites were identified usingMatInspector and compared with SiteGA results. They were
subsequently examined for the expression in the mammary gland as well as for their occurrence in the previously identified region of
homology. Finally, CCCTC binding factor (CTCF) and nuclear receptor subfamily 2 at a similar distance from the transcription start
site in the 5’ butyrophilin gene across the species. However, the exact interaction of these transcription factors with the butyrophilin
gene is not known, which needs further investigation.
demieaschalew@gmail.com