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Page 40
Volume 2
Journal of Medical Biotechnology
Biotechnology 2018
July 16-17, 2018
World Biotechnology Congress
July 16-17, 2018 Berlin, Germany
J Med Biotechnol 2018, Volume 2
Biochemical and molecular characterization of some Papaver species from Iran, a valuable potency for
producing of Morphinan alkaloids
Mohammad Reza Naghavi
University of Tehran, Iran
T
here are 150 species in the genus of
Papaver
in the world which 28 of them are natives of Iran and five of them are endemic
distributed in most part of Iran. Phytochemical evaluation of some
Papaver
species such as
Papaver bracteatum
,
Papaver
somniferum
and
Papaver orientale
from Iran revealed valuable metabolites such as noscapine, morphine; codeine; thebaine and
papaverine that mainly are used in medicine and pharmacy. The accumulation of morphinan alkaloids was different among organs
of each species. However, we found inverse relations among various metabolites which suggest a competition among biosynthetic
pathways for intermediates or organs for accumulation of final products. In addition, we found common morphinan biosynthesis
pathway in different Papaver species as what reported in
Papaver
somniferum using quantitative PCR. Relative gene expression of
important genes
(TYDC, BBE, SAT, COR, T6ODM, and CODM)
in morphinan biosynthesis pathway was highly variated depend on
growth, developmental stages and alkaloid content of the species. Our results also indicated that the elicitation of
Papaver
cell culture
by hormonal and nano elicitors can be a good system for enhancement the production of the alkaloids. In addition, our findings
suggested that inducing the hairy roots in
Papaver
species can be used as a good source for producing commercial alkaloids.
mnaghavi@ut.ac.ir