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Page 41
Volume 2
Journal of Medical Biotechnology
Biotechnology 2018
July 16-17, 2018
World Biotechnology Congress
July 16-17, 2018 Berlin, Germany
J Med Biotechnol 2018, Volume 2
Development of chestnut (
Castanea sativa Mill
.) micro-propagation through zygotic embryogenesis
Tamari Kutchava, Eka Khurtsidze and Mariam Gaidamashvili
Tbilisi State University, Georgia
To establish an effective protocol for plant regeneration through zygotic embryogenesis effects of explant, culture media and plant
growth regulators on chestnut (
Castanea sativa Mill
.) regeneration were investigated. Explant (zygotic embryo), two different media
Murashige and Skoog medium (MS) and Woody Plant Medium (WPM), different plant growth regulators (6-benzylaminopurine
(BAP), Indole-3-Butyric Acid (IBA) with different concentration (0.1, 0.2 and 3.0 mgL
-1
) and phenol inhibitors (activated charcoal,
citric acid, Polyvinylpyrrolidone (PVP)) for shoot and root induction were chosen. The culture of chestnut showed the better initiation
and multiplication rates in WPM medium. 0.1 mgL
-1
BAP in combination with activated charcoal was the best growth regulator for
shoot elongation. WPM supplemented with 0.1 mgL-1 BAP and citric acid was the best shoot proliferation. Rooting of
in vitro
plantlets was achieved onWPMmedium supplemented with 3.0 mgL
-1
IBA showing 60% intensity and was the most efficient in terms
of secondary root production. For acclimatization rooted plants were transferred to plastic pots filled with mixture of sphagnum
peat:perlite at the ratio 2:1. This protocol provides a basis for future studies on protection of rare and endangered plant species using
biotechnological approaches, thus preserving diversity of the forest ecosystems.
Tamari.kutchava2013@ens.tsu.edu.ge