3 / 16
Page 13
Journal of Neurology and Clinical Neuroscience | Volume: 03
8
th
International Conference on
NEUROLOGICAL DISORDERS,
CENTRAL NERVOUS SYSTEM AND STROKE
&
International Conference on
NEUROLOGY AND NEUROSURGERY
December 04-05, Dubai, UAE
Joint event on
J Neurol Clin Neurosci, Volume: 03
Investigation of the protective effect of Heparin pre-treatment on Cerebral Ischaemia
in Gerbils
QingShan Ye
People's Hospital of Ningxia Hui, China
T
he interruption of cerebral blood circulation may cause
stroke characterized by high Neurological Deficits
(NDs) as a result of neuronal dysfunction or destruction.
Heparin may exert a neuroprotective effect against cerebral
ischaemia/reperfusion injury. Objective: The objective of
this study was to investigate the mechanism underlying
the effects of heparin pre-treatment on cerebral injury in
the gerbil. Materials and methods: A total of 80 healthy
Mongolian gerbils were randomly divided into four groups
to establish cerebral ischaemia model by bilateral carotid
artery occlusion: control (no anaesthesia and surgery),
sham (no occlusion), non-anticoagulation (occlusion), and
anti-coagulation treatment groups (50IU/100g heparin
pre-treated, occlusion). Gerbils were anesthetized with
40mg/kg pentobarbital sodium through intraperitoneal
injection before operation except for the control group.
Then, the ND and Histopathological Damage (HD) scores
were determined. The percentage of Tumour Necrosis
Factor (TNF)-a- and interleukin (IL)-1b-positive cells were
calculated based on immunohistochemical results. The
mRNA and protein levels of caspase-9, caspase-8, FasL, and
calpain were evaluated with real-time Polymerase Chain
Reaction (RT-PCR) and western blotting, respectively.
Results: Compared with non-anticoagulation group, heparin
pre-treatment (50IU/100g) delayed the onset of dyspnoea
(p<0.05), and showed a significant decrease in ND (p<0.01),
mortality rate (p<0.05), HD (p<0.01) and percentage of
positive cells for TNF-a, IL-1b (p<0.01) in cerebral ischaemia
gerbils. Besides, the expression levels of caspase-9, caspase-8,
FasL, and calpain were reduced after pre-treatment with
50IU/100g heparin. Discussion and conclusions: The damage
caused to gerbil brain was reduced upon pre-treatment with
heparin, possibly through the amelioration of neuronal cell
apoptosis and expression of TNF-a and IL-1b. These findings
are expected to provide a new breakthrough in the study and
treatment of cerebral ischaemia.
Biography
QingShan Ye has done thousands of cases of anesthesia work, no patients
have adverse reactions and complications. The resuscitation anesthesia
of a large number of critically ill patients was successfully organized.
By adhering to the principle of "never give up" until the last minute, a
large number of patients were pulled back from death. He is a high-level
professional and technical talent of Ningxia hui autonomous region, the
director of the medical quality control center of Ningxia anesthesiology,
and the person in charge of the construction of the national key clinical
anesthesia department of the health department of People's Hospital of
Ningxia Hui Autonomous Region.
e
:
yeqingshan@hotmail.com