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Journal of Neurology and Clinical Neuroscience | Volume: 03
8th International Conference on
NEUROLOGICAL DISORDERS,
CENTRAL NERVOUS SYSTEM AND STROKE
&
International Conference on
NEUROLOGY AND NEUROSURGERY
December 04-05, Dubai, UAE
Joint event on J Neurol Clin Neurosci, Volume: 03
Alteration of extracellular matrix molecules in the developing mouse Brainstem
András Birinyi
University of Debrecen, Hungary
S everal studies have demonstrated that molecular and
structural composition of the Extracellular Matrix
(ECM) in the central nervous system undergoes profound
transformation during embryonic and early postnatal
development. The aim of this study was to detect the
changes of staining pattern of different ECM molecules in
the developing mouse brainstem by using histochemical
Wisteria Floribunda Agglutinin (WFA), Hyaluronic Acid
Probe (HA) and immunohistochemical (aggrecan,
neurocan, versican (GAG beta), TN-R and HAPLN1)
methods.
We found that HA, neurocan and versican reactions
showed diffuse neuropil staining at very early embryonic
stage (E13.5), but the Perineuronal Net (PNN) composed
of these molecules were observed only postnatally (P7).
We could not find any aggrecan, WFA or HAPLN1 staining
before birth. Postnatally WFA and aggrecan established
PNN in the reticular formation and in the vestibular and
other brainstem nuclei. Postnatally WFA, aggrecan and
HAPLN1 were restricted to the neuropil of some brainstem
nuclei, in contrast to HA, neurocan and TN-R which were
found throughout the brainstem.
Our results show that at early stages of development only a
diffuse staining of ECMmolecules is present in the neuropil
of the brainstem. The formation of a definitive PNN is
recognizable postnatally and fully developed in two weeks
old animals. We detected spatiotemporal differences in
the distribution of different ECM molecules both in the
neuropil and perineuronal net in various brainstem areas.
The pattern of ECM expression appears to be related to
the functional maturation of brainstem neural circuits,
including developmental processes such as neurogenesis,
synaptogenesis or synaptic plasticity.
Biography
András Birinyi gives lectures, seminars and practices in gross anatomy,
histology and embryology to medical and dentist students, pharmacists,
as well as physiotherapists. His scientific activities are related to the field
of quantitative morphology and neurochemistry by using intracellular
and fluorescent labelling of neurons and investigating them with light,
confocal and electron microscope. He studied the morphology of motor
related neuronal circuits in the spinal cord and brainstem of different
amphibian and mammalian species.
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